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cd14 microbeads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd14 microbeads
    Cd14 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 4731 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd14+microbeads/CD14+MicroBeads%2C+human/pm42886733-69-12-14
    Average 98 stars, based on 4731 article reviews
    cd14 microbeads - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Isolation:

    Article Title: IL-38 limits alloreactivity through modulating myeloid and T-cell activation
    Article Snippet: .. Alternatively, macrophages were differentiated from monocytes isolated with human CD14 Microbeads (Miltenyi Biotec). .. Cells were cultured in RPMI 1640 media (penicillin (100DU/mL), streptomycin (100 μg/mL), FCS (10%)) supplemented with recombinant human M-CSF (50 ng/ml, Immunotools) for 3 and 7 days.

    Article Title: Naringin promotes cancer immunity by inducing cross-presentation by antigen-presenting cells
    Article Snippet: Isolation of human CD14+ monocytes and differentiation of Ms and dendritic cells CD14+ peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral blood of healthy consenting donors using Lymphoprep (04-03-9391/03; STEMCELL Technologies, Vancouver, Canada). .. Human CD14 microbeads (130-050-201; Miltenyi Biotec B.V. & Co. KG) were used for isolation. .. CD14+ PBMCs were cultured in medium composed of 50% (v/v) AIM-V (12055-091; Gibco, Life Technologies Corp., Carlsbad, CA, USA) and 50% (v/v) RPMI-1640 medium supplemented with 10% FBS and 1% P/S.

    Article Title: Metabolically encoded cell-surface glycoRNA remodeling enhances antitumor immunity
    Article Snippet: Peripheral blood mononuclear cells were isolated from buffy coats by Ficoll-Paque density gradient centrifugation. .. CD14+ monocytes were isolated by positive selection using human CD14 MicroBeads (Miltenyi Biotec, Cat# 130-050-201) according to the manufacturer’s instructions and cultured in RPMI-1640 containing 10% fetal bovine serum, 20 ng/mL recombinant human GM-CSF and 10 ng/mL recombinant human IL-4, with half-medium changes on days 3 and 5. .. Day-7 moDCs were transfected with the indicated RNA preparations (native, PNGase F-treated, RNase cocktail-treated, or PNGase F plus RNase cocktail-treated, prepared as described above; 500 ng per well in 24-well plates) using Lipofectamine 3000 for 24 h; untreated moDCs and LPS (100 ng/mL, Sigma-Aldrich, Cat# L2630)-treated moDCs served as negative and positive controls.

    Article Title: Integrative phenotypic and transcriptomic validation of an alveolar-like macrophage model reveals early host–pathogen dynamics during Aspergillus fumigatus infection
    Article Snippet: Mononuclear cells were isolated by density-gradient centrifugation using Histopaque (density 1.077 g/mL; Sigma) and subsequently quantified using a Vi-Cell XR counter. .. Using a positive selection with human CD14 Microbeads (Miltenyi Biotec) from PBMCs according to manufacturer’s protocol, CD14 + monocytes were isolated. .. Differentiation into GM-Ms was achieved by supplementing Macrophage Serum-free medium (M-SFM) (Gibco) with three doses of 20 ng/mL GM-CSF (Miltenyi), while ALMs were generated by supplementing M-SFM with three doses of 100 ug/mL Curosurf (Chiesi), 10 ng/mL GM-CSF, 5 ng/mL IL-10 (Miltenyi) and 5 ng/mL TGF-β (Miltenyi) on day 0, 2, and 4.

    other:

    Article Title: Selective blockade of γc pathway ameliorates alopecia areata in a humanized murine model.
    Article Snippet: Alopecia areata (AA) is a chronic autoimmune disease characterized by sudden patchy hair loss and persistent inflammation.. To date, only JAK inhibitors have been approved for AA treatment, but FDA-issued black box warnings highlight the need for alternative therapies.. We previously reported that an anti-γc antibody, hC2, inhibits autoreactive B, T, and NK cells by selectively attenuating JAK/STAT signaling induced by six γc cytokines, without affecting off-target TEC kinase pathways.

    Selection:

    Article Title: Metabolically encoded cell-surface glycoRNA remodeling enhances antitumor immunity
    Article Snippet: Peripheral blood mononuclear cells were isolated from buffy coats by Ficoll-Paque density gradient centrifugation. .. CD14+ monocytes were isolated by positive selection using human CD14 MicroBeads (Miltenyi Biotec, Cat# 130-050-201) according to the manufacturer’s instructions and cultured in RPMI-1640 containing 10% fetal bovine serum, 20 ng/mL recombinant human GM-CSF and 10 ng/mL recombinant human IL-4, with half-medium changes on days 3 and 5. .. Day-7 moDCs were transfected with the indicated RNA preparations (native, PNGase F-treated, RNase cocktail-treated, or PNGase F plus RNase cocktail-treated, prepared as described above; 500 ng per well in 24-well plates) using Lipofectamine 3000 for 24 h; untreated moDCs and LPS (100 ng/mL, Sigma-Aldrich, Cat# L2630)-treated moDCs served as negative and positive controls.

    Article Title: Integrative phenotypic and transcriptomic validation of an alveolar-like macrophage model reveals early host–pathogen dynamics during Aspergillus fumigatus infection
    Article Snippet: Mononuclear cells were isolated by density-gradient centrifugation using Histopaque (density 1.077 g/mL; Sigma) and subsequently quantified using a Vi-Cell XR counter. .. Using a positive selection with human CD14 Microbeads (Miltenyi Biotec) from PBMCs according to manufacturer’s protocol, CD14 + monocytes were isolated. .. Differentiation into GM-Ms was achieved by supplementing Macrophage Serum-free medium (M-SFM) (Gibco) with three doses of 20 ng/mL GM-CSF (Miltenyi), while ALMs were generated by supplementing M-SFM with three doses of 100 ug/mL Curosurf (Chiesi), 10 ng/mL GM-CSF, 5 ng/mL IL-10 (Miltenyi) and 5 ng/mL TGF-β (Miltenyi) on day 0, 2, and 4.

    Cell Culture:

    Article Title: Metabolically encoded cell-surface glycoRNA remodeling enhances antitumor immunity
    Article Snippet: Peripheral blood mononuclear cells were isolated from buffy coats by Ficoll-Paque density gradient centrifugation. .. CD14+ monocytes were isolated by positive selection using human CD14 MicroBeads (Miltenyi Biotec, Cat# 130-050-201) according to the manufacturer’s instructions and cultured in RPMI-1640 containing 10% fetal bovine serum, 20 ng/mL recombinant human GM-CSF and 10 ng/mL recombinant human IL-4, with half-medium changes on days 3 and 5. .. Day-7 moDCs were transfected with the indicated RNA preparations (native, PNGase F-treated, RNase cocktail-treated, or PNGase F plus RNase cocktail-treated, prepared as described above; 500 ng per well in 24-well plates) using Lipofectamine 3000 for 24 h; untreated moDCs and LPS (100 ng/mL, Sigma-Aldrich, Cat# L2630)-treated moDCs served as negative and positive controls.

    Recombinant:

    Article Title: Metabolically encoded cell-surface glycoRNA remodeling enhances antitumor immunity
    Article Snippet: Peripheral blood mononuclear cells were isolated from buffy coats by Ficoll-Paque density gradient centrifugation. .. CD14+ monocytes were isolated by positive selection using human CD14 MicroBeads (Miltenyi Biotec, Cat# 130-050-201) according to the manufacturer’s instructions and cultured in RPMI-1640 containing 10% fetal bovine serum, 20 ng/mL recombinant human GM-CSF and 10 ng/mL recombinant human IL-4, with half-medium changes on days 3 and 5. .. Day-7 moDCs were transfected with the indicated RNA preparations (native, PNGase F-treated, RNase cocktail-treated, or PNGase F plus RNase cocktail-treated, prepared as described above; 500 ng per well in 24-well plates) using Lipofectamine 3000 for 24 h; untreated moDCs and LPS (100 ng/mL, Sigma-Aldrich, Cat# L2630)-treated moDCs served as negative and positive controls.

    Derivative Assay:

    Article Title: Rejuvenated Hematopoietic Stem and Progenitor Cell-Engineered CAR-Armored Natural Killer T Cells for Malignant Pleural Mesothelioma
    Article Snippet: .. Note that these myeloid cells were derived from the same donor and purified by MACS using human CD14 MicroBeads (Miltenyi Biotech, cat. no. 130-097-052) to remove non-myeloid populations. ..

    Purification:

    Article Title: Rejuvenated Hematopoietic Stem and Progenitor Cell-Engineered CAR-Armored Natural Killer T Cells for Malignant Pleural Mesothelioma
    Article Snippet: .. Note that these myeloid cells were derived from the same donor and purified by MACS using human CD14 MicroBeads (Miltenyi Biotech, cat. no. 130-097-052) to remove non-myeloid populations. ..

    Magnetic Cell Separation:

    Article Title: Rejuvenated Hematopoietic Stem and Progenitor Cell-Engineered CAR-Armored Natural Killer T Cells for Malignant Pleural Mesothelioma
    Article Snippet: .. Note that these myeloid cells were derived from the same donor and purified by MACS using human CD14 MicroBeads (Miltenyi Biotech, cat. no. 130-097-052) to remove non-myeloid populations. ..

    Article Title: Particulate matter 2.5 induced inflammatory responses and metabolic alterations in human monocytes.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coat or whole blood of healthy donors by Ficoll gradient centrifugation (GE Healthcare, UK). .. PBMCs was used as sources for CD14+ monocytes separation by human CD14 microbeads (MACS Miltenyi Biotec, Germany). .. CD14+ monocytes were maintained in RPMI-1640 supplemented with 5% human serum (Sigma, USA) and antibiotics plus antimycotics (Gibco, USA).



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